Wednesday, 9 August 2023

Classification of fungi

      1.6 Classification of fungi

     In your previous classes, I assured you that you have learnt about the classification of living organisms. You may remember that in the most precise words classification is the grouping of organisms in particular taxa on the basis of their most similar characters. It gives information about how an organism is similar or dissimilar from other organisms. However, classification has three functions –

        i) It provides a framework of recognizable features by which an organism under examination can                 be identified,

        ii) It is an attempt to group together organisms that are related to each other, and

        iii) It assists in the retrieval of information about the identified organisms in the form of a list or

                catalogue.

         Today I am going to discuss about classification of fungi.

     In the above, we have discussed different aspects of fungi such as cell structure, thallus structure, mode of nutrition, etc. There, we learnt some of the characteristics that are different from the other plants. Some are different from animals. You might also be able to explain some characters have affinities with plants and some have affinities with animals. Therefore, such characteristics are reasons to place fungi in another group in the classification system.

    R. H. Whittaker (1969) proposed a five-kingdom system of classification in which fungi were placed in separate groups. He kept them in the kingdom “Fungi” considering all the multicellular eukaryotic fungi with a few unicellular fungi (Eg. Yeast). Likewise, different authors have attempted to classify fungi most appropriately at their level best. But, still, no concrete classification module of fungi has been fixed. However, here, I am going to discuss the two most acceptable forms of fungal classification. 

  In earlier days fungi were classified on the basis of some morphological and microscopic characteristics such as –

a) Differentiation into plasmodium or mycelium,

b) Presence or absence of septa,

c) Structure and production of sexual spores,

d) Nature of reproductive structure or fruiting bodies etc.

    The classification based on such characters may be called a traditional system of fungal classification. In such a classification system, characters were elaborated by the observations and identified the fungi on the basis of observed characters. In such a classification system, phylogenetic characters were not studied and, so sometimes related species were kept in another group or apart. Even, non-fungal members have been included in the kingdom of fungi. Such a classification system was dominant till the middle of twenty century.

  From the later part of the twentieth century, new approaches in fungal taxonomy have evolved with inputs from recent technologies, ultrastructure studies, DNA and RNA sequencings, biochemical analysis and new phylogenetic systems have come up.

 Like other groups of plant classification, fungal taxonomy and nomenclature are also governed by the International Code for Botanical Nomenclature (ICBN).

Fungi classification can be categorised as Traditional classification and Modern classification. 

1.6.1 Traditional methods of classification

        The traditional methods of fungal classifications can be considered the works carried out by Bessey (1950), G. W. Martin (1961), C. J. Alexopoulos (1962) and G. C. Ainsworth (1971). The basic outline of their classifications is given below. Here, we are going to discuss in detail of traditional classification done by C. J. Alexopoulos (1962) and G. C. Ainsworth (1971). 

1.6.1 (a) Bessey (1950)

              Let us discuss the old traditional classification system of fungi proposed by Bessey in 1950. As I read he divided true fungi into lower and higher fungi and then into classes on the basis of the extent of thallus development, life cycle patterns, and flagellation of reproductive structures. Just remember that he simply classified the fungi into two groups –

          i) Lower fungi,

          ii) Higher fungi

 i) Lower fungi group contained

Class I: Phycomycetes. He placed fungal members in this class on the basis of the following characteristics  –

     a) Unicellular thallus,

    b) Coenocytic mycelium,

    c) Sexual reproduction forms oospore or zygospore

ii) Higher fungi: The fungi having one celled, not producing planocytes or producing cellular mycelium were placed under this group. This group is divided into three classes –

Class I : Ascomyceteae – Fruiting body is ascus,

Class II: Basidiomyceteae – Fruiting body is basidium,

Class III: Fungi Imperfecti – Sexual reproductive stage not known

Bessey did not consider Myxomycetes as fungi. so he placed them under the name Mycetozoa outside the limits of the vegetable kingdom.  Let us see the classification outline of Bessey (1950) in Figure 1.


Figure 1. Outline of Bessey (1950) fungal classification

1.6.1 (b)  G. W. Martin (1961)

       From the Bessy classification, you might get an idea of how fungi were classified on the basis of a few characters. But you remember that one classification process could not satisfy another as knowledge is growing up among researchers every day. So from the esteemed end of every researcher, they try to give their best classification system and still, the same working trend is going on.

      Let us see G. W. Martin and how he classified the fungi into different divisions or classes etc. 

     In his classification system, he included slime moulds in the fungi. He divided the division fungi Mycota into two subdivisions. 

He had divided the entire fungi (Mycota) group into two sub-division-

1. Sub-division: Myxomycotina – in this sub-division, he included all slime moulds,

2. Sub-division: Eumycotina – in this sub-division, he included the true fungi.                                                                                   

1. Sub-division- Myxomycotina – This sub-division contained one sub-class, 5- orders,

2. Sub-division – Eumycotina – This sub-division contained 4 classes

i) Class I- Phycomycetes with 3 sub-classes and 16 orders,

ii) Class II – Ascomycetes with 2 sub-classes and 25 orders,

iii) Class III- Basidiomycetes with 2 sub-classes and 11 orders

iv) Class IV- Deuteromycetes with no sub-class and 4 orders.

       Now, let us see the classification outline of  W. Martin (1961) in Figure 2.


Figure 2. Outline of, G. W. Martin (1961), the traditional system of fungal classification 1.6.1 (c) C.  J.Alexopoulos (1962)

Now, I am going to discuss the Alexopoulos (1962) classification system. From the previous two classification systems, namely Bessey and Martin, you have observed the developing trends of classification methods. Subsequent workers got more exposoure to incorporate new characters in their classification system. Gradually classification became more updated. Again, we are going to discuss the little more advanced classification method that was forwarded by C. J. Alexopoulos in 1962.

   Alexopoulos first divided the Mycota into two sub-division. Do you remember who has done it so likely? His two sub-division were 1. Myxomycotina and 2. Eumycotina. In the sub-division Myxomycotina, he included all the fungi having no wall in the hyphae and in the sub-division Eumycotina, he included fungi that have walls and true fungi.

     Sub-division Myxomycotina has only one class, namely Myxomycetes, while sub-division Eumycotina has 8 classes and one form class.

    Let us see the classification outline of Alexoulos (1962) classification in Figure 3.


Figure 3. Outline of C. J. Alexopoulos (1962) classification

However, in 1979 Alexopoulos with C. W. Mims modified the previous classification. They removed fungi from the kingdom Plantae and gave them a kingdom status called Myceteae which was further divided into Divisions, Sub-division, Classes, etc. They included all fungi including slime moulds in the kingdom Myceteae of the super kingdom Eukaryonta.

     You may go for further study of the modified classification proposed by C. J. Alexopoulos and  C. W. Mims (1979).

1.6.1 (d)   G. C. Ainsworth (1971)

      Now, we are going to discuss latest traditional classification proposed by G. C. Ainsworth in 1971. He has published his this claasification method in his book “Dictionary of Fungi”. This classification is based on the following features –

a) Presence or absence of cell wall,

b) Presence or absence of motile cells,

c) Nature and type of asexual spores,

d) Number of flagella and their position on the zoospores,

e) Nature of sporophores,

f) Presence and absence of fruiting bodies and their nature of the present.

         Let us see brief outline (Figure 4)) and in details outline (Figure 5.) of G. C. Ainsworth (1971) classification.


 Figure 4. A brief outline of explanation of G. C. Ainsworth (1971) classification


Figure 5. Outline of G. C. Ainsworth (1971) classification


       Check Your Progress

     a) According to your understanding which traditional classification is most

         acceptable and why?

     b) How Ainsworth classification is best among the other classification methods.

         justify it?

     c) Why term traditional is used in fungal classification?


1.6.2 Modern classification

                  Dear students, now I am going to discuss the modern classification of fungi. Before going to the topic, we would like to review the traditional methods of fungal classification. I hope you are now able to explain about traditional classifications methods of fungi that forward by different workers. Starting from the Bessey (1950) to Ainsworth (1971)  of different traditonal methods of fungal classification, we have seen all the classification systems were based on basic characteristics such as cell structure, present or absence of wall, presence or absence of motile cell, nature of asexual and sexual spores, number of flagella and position, unicellular cell structure, etc. In traditional classification methods, phylogenetic relationships among the different groups were ignored. Even, non-fungal members were also included in the fungal groups or fungal groups were kept apart from fungi. In traditional classification, we have not seen any genetic or biochemical characteristics among the same or different groups.

      To overcome the demerits of traditional classification, with the aids of modern technologies, some researchers were forwarded the modern classification of fungi.

        As we discuss in previous classes from the later part of the twentieth century, new approaches in fungal taxonomy have evolved with inputs from recent technologies, ultrastructure studies, DNA and RNA sequencings, biochemical analysis and new phylogenetic systems have come up.  However, the traditional methods are not ignored as they provide detailed descriptions and morphological features of different species and groups. In this section, we are going to discuss the modern classification of fungi.

Therefore, considering the confusion in the groups, deviation from the ICBN rules, and the new features gained from the molecular phylogeny, several workers have attempted in a coordinated manner since the last decade of the twentieth century to decide the phylogenetic classification of fungi with a broad scope by formulating the following principles –

i) Classification should not be misleading about the phylogeny of the organisms they represent,

ii) Methods of classification should efficiently promote the translation of phylogenetic hypotheses into classification. It says that once a monophyletic group has been discovered by a systematist, it should be possible to name it quickly and simply,

iii) Classification should be stable.

        Lets us discuss some classification methods based on these principles which are considering modern classification –

 

1.6.2(a) Webster and R.W.S. Weber (2007)

                 Let us discuss one of the most recent classifications that put forwarded by Webster and R.W.S. Weber in 2007 and published in the 3rd edition of their book “Introduction to Fungi”. They have included the slime moulds in the division Myxomycota under Protozoa kingdom.  Now we are going to see the outlines of their classification –

     According to their classification, the fungi and fungus-like organisms have been segregated in the three kingdoms Protozoa, Straminipila and Eumycota.

Under the kingdom Protozoa – there are 2 divisions which contain unicellular fungi-like organisms. These 2 divisions are –

Division 1: Myxomycota: It has 4 classes - i) Acrasiomycetes, ii) Dictyoteliomycetes, iii) Protosteliomycetes, and iv) Myxomycetes.

Division 2: Plasmodiophoromycota: It has 2 orders Plasmodiophorales and Haptoglossales.

Under the kingdom Starminipila – there are 3 divisions.

Division 1: Hyphochytriomycota,

Division 2: Labyrinthalomycota,

Division 3: Oomycota with 8 orders.

Under the kingdom Eumycota – there are 4 divisions.

Division 1: Chytridimycota with 1 class (Chytridiomycetes)

Division 2: Zygomycota with 2 classes (Zygomycetes and Trichomycetes)

Division 3: Ascomycota with 5 classes (Archiascomycetes, Hemiascomycetes,

                   Plectomycetes, Hemenoascomycetes and Ustilaginomycetes).

Division 4: Basidiomycota with 4 classes (Homobasidiomycetes,

                   Heterobasidiomycetes, Urediniomycetes and Ustilaginomycetes).

 

1.6.2 (b) D. S. Hibbett et. al.  (2007)

     Let us discuss another most recent method of fungal classification that was forwarded by Hibbett and his team in 2007. This is a comprehensive phylogenetic classification of fungi. The basic features of their classification are –

a) Fungi and animals are considered sister groups. They have closed relatives and share a common ancestor known as the opisthokont clade,

b) The phylum Zygomycota is replaced by the term Glomeromycota. Because phylum Zygomycota is not accepted in the most recent classification because doubt raised about the relationships between the groups that have been traditionally placed in this phylum,

c) They have treated evolutionary characters of different groups in their classification. Aquatic final groups such as Chytridiomycota are considered to be the most primitive,

d) They expressed that the evolution of higher fungi took place first by the loss or withdrawal of chytrid flagellum and the development of branching, aseptate fungal filaments,

e) Septate filaments evolved by divergence from the Glomeromycota as a combined pre-Basidiomycota or pre-Ascomycota clade about 500 million years ago,

f) They considered Basidiomycota and Ascomycota as sister groups that diverged about 300 million years ago and hence placed under the sub-kingdom Dikarya,

g) Aerobic chytrids have given rise to symbiotic fungi residing in the lumens of grazing mammals helping in their digestion recently perhaps 60-80 million years ago,

h) The members whose phylogenies have not been ascertained are placed in that class or phylum,

i) They have separated the pseudo-fungi-like organisms from the true fungi and classified them under the sub-kingdom Chromista and sub-kingdom Protozoa,

j) The traditional class Phycomycetes have been removed and similarly Myxomycetes of traditional classification is considered under the kingdom Protozoa,

k) True fungi which make up the monophyletic clade are called Kingdom Fungi and have been classified into 7 phyla.

 Now, in our discussion, we are going to discuss only the kingdom of Fungi. As we are now aware that kingdom fungi have been divided into 7 phyla by Hibbett et. al.  (2007)

 

   They have divided the kingdom of fungi into 7 phyla.  

 

Phylum 1: Chytridiomycota with 2 classes, 4 orders

     Water moulds and aquatic fungi produce motile zoospores, simple posterior flagellum, unicellular/ filamentous, holocarpic/eucarpic, and zygotic meiosis, considered most ancestral.

 

Phylum 2: Neocallimastigomycota with 1 class and 1 order

     The thallus mono/polycentric, anaerobic, found in digestive systems of large herbivores mammals, and zoospores positively uniflagellate/polyflagellate.

 

Phylum 3: Blastocladiomycota with 1 class and 1 order

     Water moulds, and aquatic fungi, all have zoospores, saprophytes, parasites on algae or plants and invertebrates, thallus monocentric/polycentric, and sometimes mycelia.

 

Phylum 4: Microsporidia

     Unicellular parasites of animals, considered as sister group of rest of fungi.


 Phylum 5: Glomeromycota with 5 sub-phylum and 9 orders

Symbiotic fungi. They do not form zygospores.

 

Phylum 6: Ascomycota with 3 sub-phylum, 15 classes and 1 order

     This is the largest group of fungi and the lifestyles adopted to cover the complete range from saprotrophs to symbionts and parasites. Sexual spores are known as ascospores formed endogenously within the ascus. Except for yeasts, asci are produced in complete fruiting bodies. The basic type of the genus is Pezzia. This group contains about 64000 species under 6355 genera.

 

Phylum 7: Basidiomycota with 3 sub-phylum, 13 classes and 47 orders

        This group of fungi produce basidiospores within the basidium. They are generally saprophytes, but parasites on plants and insects. Thallus filamentous with septate hyphae. Septa have central pores which are called dolipore. The filaments are 2 types – primary homokaryotic (having 1 nucleus) and secondary heterokaryotic with 2 nucleus or dikaryotic cells. Asexual takes place by fragmentation of the thallus, oidia, conidia etc. Sexual reproduction performs by somatogamy and basidiospores. It contains about 1600 genera and 32000 species.

 

  Check Your Progress

a) Why modern classification methods are brought up by various mycologists?

b) How modern classification methods are more reliable than traditional ones?

c) Among the modern classification methods, which one will you refer to for your

    better understanding?

d) What informations are being carried out in the word “Phylogeny”?

e) How phylogeny helps in classification?


Figure 7: Outline of fungal classification forwarded by D. S. Hibbett et. al.  (2007)


1.7 Let us sum up-

     Dear students, we have discussed several aspects of fungi in your introductory class. From our discussion, we are now in a position to define fungi, explained to thallus organization of fungi, and fungal nutrition and later part of the unit we have discussed both traditional and non-traditional methods of fungal classification.

     It is again reminding you that fungi are heterotrophic organisms. They have certain affinities with plants and animals. But from the point of plant taxonomy, they differ from both plants and animals.  Due to their enormous similarity with plants, they are placed under the plant kingdom. Like plants, they have cells, which is the major difference from animal cells. We have discussed that fungi draw their nutrition in different ways. Some fungi draw their nutrition from dead and decaying organic matter – called saprophytes; some fungi draw their foods from living organisms – called parasites; some fungi live by sharing foods and other elements with organisms in mutual benefits- called symbiosis and some fungi prey on other organisms – called as predacious.

     In the classification section, we have discussed different methods of fungal classifications forwarded by different authors. I assured you that you are now able to explain traditional and non-traditional methods of fungal classification. In traditional methods, only external characters were considered for grouping the fungi. In such classification methods, taxonomists ignored phylogenetic relationships among the different groups. To overcome such problems, in recent years mycologists have classified the fungi by adding the phylogenetic characters apart from characters used in traditional methods. The most recent modern classification methods are forwarded by Webster and R.W.S. Weber (2007), D. S. Hibbett et. al.  (2007), etc.

For more study, you may go through the latest editions of mycology books.

1.8 Glossary –

Chitin: a complex polysaccharide, a polymer of N-acetylglucosamine, found in      the exoskeletons of arthropods and in the cell walls of fungi; thought to be responsible for some forms of asthma in humans

 

Hypha: a long, branching, filamentous structure of a fungus that is the main

             mode of vegetative growth

Mycelium: the vegetative part of any fungus, consisting of a mass of branching,                 threadlike hyphae, often underground

 

Thallus: the vegetative body of a fungus

 

Saprophyte: any organism that lives on dead organic matter, as certain fungi and bacteria

 

 Septum: cell wall division between hyphae of a fungus


1.9 Further readings

1. Alexopoulos, C.J., Mims, C.W., Blackwell, M. (1996). Introductory Mycology,

    John Wiley & Sons (Asia) Singapore. 4th edition

2. Webster, J. and Weber, R. (2007). Introduction to Fungi, Cambridge University

    Press, Cambridge. 3rd edition.


 Answer to check your progress-

 

1. What do you mean by heterotropic mode of nutrition?

Ans- The organisms which cannot prepare their foods and depends on other sources  

         are called the heterotrophic mode of nutrition. Example – Man, Fungi etc.

 

2. What is the saprophytic mode of fungal nutrition?

Ans- The fungal groups who draw their nutrition from dead and decaying organic

        material is called saprophytic fungi and this mode of nutrition is saprophytic

         mode of nutrition.

 

3. What are the characters considered in traditional classification methods?

Ans- In traditional classifications methods superficial characters such as vegetative

        structures, hyphal structure, structure and number of reproductives bodies are

        considered. 

Bibliography

1. Alexopoulos, C.J., Mims, C.W., Blackwell, M. (1996). Introductory Mycology,

    John Wiley & Sons (Asia) Singapore. 4th edition

2. Webster, J. and Weber, R. (2007). Introduction to Fungi, Cambridge University

    Press, Cambridge. 3rd edition.

3. Sethi, I.K. and Walia, S.K. (2011). Textbook of Fungi and Their Allies,

    Macmillan Publishers India Ltd.

5. Sharma, P.D. (2011). Plant Pathology, Rastogi Publication, Meerut, India.   

 

Probable Questions

       Very short questions-

      1. What is the reserve food materials of fungi?

      2. How many fungal groups are placed in the Bessey classification?

      3. Why fungus body is called thallus?

      4. Who has forwarded the phylogenetic classification of fungi?

 

    Short questions (Write within 100 – 150 words)

       1. Write the principles of modern fungal classifications?

       2. Write the different modes of fungal nutrition?

       3. Write a note on fungal cell wall compositions?

       4. Write the thallus organisation in fungi? 

 

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